Fluorometric Method for Microdetermination of Human Serum Albumin Using Substituted 3H-Indole Compound

Binding of Indole Analogues to Human Serum Albumin

In earlier studies on the binding of indole analogues with defatted human serum albumin (l), the intrinsic association constants for both a neutral ligand and a ligand bearing a negative charge were found to be invariant in the pH region of 5 to 10, implying that the structure of the binding site on the albumin did not change in this region. As the pH increased or decreased from this region the...

Photoactivated covalent binding of [3H]bilirubin to human serum albumin.

A one-step procedure has been developed for the preparation of [3H]bilirubin IX-alpha in good yield from unlabelled bilirubin. Irradiation of an aqueous solution of [3H]bilirubin IX-alpha in the presence of human serum albumin results in the covalent attachment of the bilirubin to the protein. Preliminary degradation studies have been carried out to locate the site of attachment of the bilirubi...

Fluoxetin Competes with Cortisol for Binding to Human Serum Albumin

Human serum albumin (HSA) is an important protein that carries variety of substances like some hormones and drugs in blood. Pharmacological studies of the interaction of many drugs and HSA are reported during several decades, specially recently years. Interaction of cortisol and fluoxetine hydrochloride (FLX) (as a common anti-stress drug) with HSA (as their carrier in blood) has been studied s...

bioinformatics analysis of human serum albumin for determination of herbal anti-diabetic compound binding site

Fluorometric method for measuring serum lipase activity.

We describe a method for determining lipase (triacylglycerol acyl-hydrolase, EC 3.1.1.3) activity in serum. Emulsified olive oil in tris(hydroxymethyl)aminomethane buffer is used as substrate. The course of the enzymatic reaction is followed by measuring the decrease in fluorescence with time of a fluorescent indicator, 4-methylumbelliferone, added to the substrate, caused by the change in the ...